Structure analysis

Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)

Electron Microscopy
2.7Å resolution
Source organism: Pseudomonas aeruginosa PAO1
Assembly composition:
hetero 57-mer (preferred)
Entry contents: 52 distinct polypeptide molecules
5 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 57-mer
Accessible surface area: 836291.19 Å2
Buried surface area: 265870.0 Å2
Dissociation area: 578.12 Å2
Dissociation energy (ΔGdiss): -3.44 kcal/mol
Dissociation entropy (TΔSdiss): 11.59 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-249485

Macromolecules

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Chain: h
Length: 130 amino acids
Theoretical weight: 14.19 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWE9 (Residues: 1-130; Coverage: 100%)
Gene names: PA4249, rpsH
Pfam: Ribosomal protein S8
InterPro:

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Chain: l
Length: 123 amino acids
Theoretical weight: 13.83 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWD0 (Residues: 1-123; Coverage: 100%)
Gene names: PA4268, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:

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Chain: n
Length: 101 amino acids
Theoretical weight: 11.59 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWE8 (Residues: 1-101; Coverage: 100%)
Gene names: PA4250, rpsN
Pfam: Ribosomal protein S14p/S29e
InterPro:

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Chain: o
Length: 89 amino acids
Theoretical weight: 10.14 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HV58 (Residues: 1-89; Coverage: 100%)
Gene names: PA4741, rpsO
Pfam: Ribosomal protein S15
InterPro:

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Chain: p
Length: 83 amino acids
Theoretical weight: 9.22 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HXP9 (Residues: 1-83; Coverage: 100%)
Gene names: PA3745, rpsP
Pfam: Ribosomal protein S16
InterPro:

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Chain: q
Length: 88 amino acids
Theoretical weight: 10.11 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWE4 (Residues: 1-88; Coverage: 100%)
Gene names: PA4254, rpsQ
Pfam: Ribosomal protein S17
InterPro:

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Chain: r
Length: 76 amino acids
Theoretical weight: 8.89 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HUN0 (Residues: 1-76; Coverage: 100%)
Gene names: PA4934, rpsR
Pfam: Ribosomal protein S18
InterPro:

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Chain: t
Length: 91 amino acids
Theoretical weight: 9.94 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HVM1 (Residues: 1-91; Coverage: 100%)
Gene names: PA4563, rpsT
Pfam: Ribosomal protein S20
InterPro:

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Chain: u
Length: 71 amino acids
Theoretical weight: 8.51 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9I5V8 (Residues: 1-71; Coverage: 100%)
Gene names: PA0579, rpsU
Pfam: Ribosomal protein S21
InterPro:

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Chain: E
Length: 200 amino acids
Theoretical weight: 21.67 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWD6 (Residues: 1-200; Coverage: 100%)
Gene names: PA4262, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:

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Chain: P
Length: 129 amino acids
Theoretical weight: 14.53 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: O52761 (Residues: 1-129; Coverage: 100%)
Gene names: PA4237, rplQ
Pfam: Ribosomal protein L17
InterPro:

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Chain: Q
Length: 116 amino acids
Theoretical weight: 12.69 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWF1 (Residues: 1-116; Coverage: 100%)
Gene names: PA4247, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:

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Chain: R
Length: 116 amino acids
Theoretical weight: 13.06 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HXQ2 (Residues: 1-116; Coverage: 100%)
Gene names: PA3742, rplS
Pfam: Ribosomal protein L19
InterPro:

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Chain: S
Length: 118 amino acids
Theoretical weight: 13.4 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9I0A2 (Residues: 1-118; Coverage: 100%)
Gene names: PA2741, rplT
Pfam: Ribosomal protein L20
InterPro:

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Chain: V
Length: 99 amino acids
Theoretical weight: 10.97 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWD7 (Residues: 1-99; Coverage: 100%)
Gene names: PA4261, rplW
Pfam: Ribosomal protein L23
InterPro:

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Chain: Y
Length: 85 amino acids
Theoretical weight: 9.01 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HVL7 (Residues: 1-85; Coverage: 100%)
Gene names: PA4567, rpmA
Pfam: Ribosomal L27 protein
InterPro:

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Chain: 1
Length: 63 amino acids
Theoretical weight: 7.21 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWE3 (Residues: 1-63; Coverage: 100%)
Gene names: PA4255, rpmC
Pfam: Ribosomal L29 protein
InterPro:

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Chain: 4
Length: 60 amino acids
Theoretical weight: 6.81 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HZN4 (Residues: 1-60; Coverage: 100%)
Gene names: PA2970, rpmF
Pfam: Ribosomal L32p protein family
InterPro:

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Chain: 6
Length: 44 amino acids
Theoretical weight: 5.23 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: P29436 (Residues: 1-44; Coverage: 100%)
Gene names: PA5570, rpmH
Pfam: Ribosomal protein L34
InterPro:

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Chain: 8
Length: 38 amino acids
Theoretical weight: 4.45 KDa
Source organism: Pseudomonas aeruginosa PAO1
UniProt:
  • Canonical: Q9HWF6 (Residues: 1-38; Coverage: 100%)
Gene names: PA4242, rpmJ
Pfam: Ribosomal protein L36
InterPro:

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Name: 16S ribosomal RNA
Representative chains: a
Length: 1536 nucleotides
Theoretical weight: 497.97 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: P-site initiator tRNA
Representative chains: v
Length: 77 nucleotides
Theoretical weight: 24.85 KDa
Rfam: tRNA

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Name: Messenger RNA
Representative chains: w
Length: 24 nucleotides
Theoretical weight: 7.8 KDa

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Name: 23S ribosomal RNA
Representative chains: A
Length: 2891 nucleotides
Theoretical weight: 937.13 KDa
Rfam: Bacterial large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 120 nucleotides
Theoretical weight: 38.6 KDa
Rfam: 5S ribosomal RNA

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