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Cryo-EM structure of histamine H1 receptor in complex with histamine and miniGq

Electron Microscopy
2.66Å resolution
PDB entry 8yn2 coloured by chain, front view.
PDB entry 8yn2 coloured by chain, side view.
PDB entry 8yn2 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero pentamer (preferred)
Entry contents: 5 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentamer
Accessible surface area: 48201.13 Å2
Buried surface area: 14941.02 Å2
Dissociation area: 949.06 Å2
Dissociation energy (ΔGdiss): 0.38 kcal/mol
Dissociation entropy (TΔSdiss): 13.56 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279919

Macromolecules

Engineered guanine nucleotide-binding protein G(q) subunit alpha
Chain: A
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: synthetic construct
Expression system: Spodoptera frugiperda
Engineered guanine nucleotide-binding protein G(q) subunit alpha in PDB entry 8yn2, assembly 1, front view.
Engineered guanine nucleotide-binding protein G(q) subunit alpha in PDB entry 8yn2, assembly 1, side view.
Engineered guanine nucleotide-binding protein G(q) subunit alpha in PDB entry 8yn2, assembly 1, top view.
124620406080100120140160180200220240
 
100200
Chains
Chain A (auth A)
Secondary structure
Interaction interfaces
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Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Chain: B
Length: 376 amino acids
Theoretical weight: 40.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Pfam: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8yn2, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8yn2, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 in PDB entry 8yn2, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P62873  
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Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Pfam: GGL domain
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8yn2, assembly 1, front view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8yn2, assembly 1, side view.
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 in PDB entry 8yn2, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P59768  
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Antibody fragment scFv16
Chain: E
Length: 255 amino acids
Theoretical weight: 27.37 KDa
Source organism: synthetic construct
Expression system: Trichoplusia ni
Antibody fragment scFv16 in PDB entry 8yn2, assembly 1, front view.
Antibody fragment scFv16 in PDB entry 8yn2, assembly 1, side view.
Antibody fragment scFv16 in PDB entry 8yn2, assembly 1, top view.
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Histamine H1 receptor
Chain: R
Length: 716 amino acids
Theoretical weight: 80.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35367 (Residues: 1-487; Coverage: 100%)
Gene name: HRH1
Pfam: 7 transmembrane receptor (rhodopsin family)
Histamine H1 receptor in PDB entry 8yn2, assembly 1, front view.
Histamine H1 receptor in PDB entry 8yn2, assembly 1, side view.
Histamine H1 receptor in PDB entry 8yn2, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P35367  
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