$VAR1 = undef;

Summary for peptidase C01.032: cathepsin L

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates Pharma

 

Names
MEROPS Namecathepsin L
Other namesSPase (Cercopithecus aethiops)
Domain architecture
MEROPS Classification
Classification Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.032
Holotypecathepsin L (Homo sapiens), Uniprot accession P07711 (peptidase unit: 113-333), MERNUM MER0000622
History Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.
Activity
Catalytic typeCysteine
PeplistIncluded in the Peplist with identifier PL00060
NC-IUBMBSubclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.15
EnzymologyBRENDA database
Proteolytic eventsCutDB database (47 cleavages)
Activity statushuman: active (Kirschke, 2004)
mouse: active (Mason et al., 1989)
PhysiologyEndopeptidase activity in lysosomal proteolysis, and probably also contributes to the generation of anigenic peptides for the MHC II system. In addition, it is reported that cathepsin L synthesised without a signal peptide localises to the nucleus, and functions in the regulation of cell cycle progression through proteolytic processing of the CDP/Cux transcription factor (Goulet et al., 2004).
KnockoutThe gene was inactivated by gene targeting in mouse embryonic stem cells (Roth et al., 2000). The deficient mice develop periodic hair loss and epidermal hyperplasia, acanthosis, and hyperkeratosis. Cathepsin L deficiency was shown to be a molecular defect of the previously-known furless mouse. Combined deficiency of cathepsins B and L in mice is lethal during the second to fourth week, and associated with a degree of brain atrophy not previously seen in mice (Felbor et al., 2002). Mice that express enzymatically inactive cathepsin L exhibit abnormal spermatogenesis (Wright et al., 2003).
Pathways KEGGAntigen processing and presentation
KEGGLysosome
KEGGPhagosome
KEGGProteoglycans in cancer
KEGGRheumatoid arthritis
Other databases WIKIPEDIAhttp://en.wikipedia.org/wiki/Cathepsin_L1
Cleavage site specificity Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern-/-/lv/-Scissile bond-/-/-/- (based on 6703 cleavages)
weblogo
Specificity matrix
 
Amino acid P4 P3 P2 P1 P1' P2' P3' P4'
Gly 485 462 30 817 468 484 623 534
Pro 354 269 147 14 8 490 603 744
Ala 575 494 280 622 965 773 697 621
Val 456 386 1250 111 347 408 355 353
Leu 435 1015 1918 340 367 317 440 335
Ile 253 352 710 64 329 361 327 254
Met 148 242 142 197 144 82 136 58
Phe 222 221 777 146 110 136 195 88
Tyr 152 147 511 120 49 101 131 81
Trp 68 54 142 44 7 17 24 12
Ser 418 400 84 537 658 515 362 395
Thr 295 301 131 548 380 410 255 332
Cys 102 81 109 94 75 72 67 42
Asn 251 265 30 218 282 249 302 277
Gln 349 282 66 530 310 347 321 290
Asp 276 163 30 204 350 418 423 731
Glu 513 313 82 534 662 711 563 778
Lys 546 750 120 906 856 614 644 609
Arg 418 319 106 532 210 89 102 73
His 146 132 32 123 112 90 115 76
Specificity from combinatorial peptides
 
Organism comment P4 P3 P2 P1 P1' P2' P3' P4' optimal substrate fluorophore or acceptor-donor pair Reference
Homo sapiens recombinant - K/R F R H/S S/H - - xKFR+HSxx Abz-EDDnp Puzer et al., 2004
Rattus norvegicus recombinant - - - - S/A/K - - - xxxx+Sxxx Dansyl-Trp M�nard et al., 1993
Homo sapiens recombinant H/broad K/R/n/M/L F/W/Y R/K - - - - HKFR ACC Choe et al., 2006
Homo sapiens recombinant - - - Iaf/Amf - - - - xxxIaf+xxxx Abz-EDDnp Alves et al., 2001
Homo sapiens recombinant - - - - R/A/K - - - xxx+Rxxx Abz-EDDnp Melo et al., 2001
Human genetics
Gene symbol Locus Megabases Ensembl Entrez gene Gene Cards OMIM
CTSL1 9q21-q22 ENSG00000135047 1514 CTSL 116880
Mouse genetics
Gene symbol Position Megabases Ensembl Entrez gene MGI
Ctsl 13:B3 ENSMUSG00000021477 13039 MGI:88564