Structure for peptidase S45.001: penicillin G acylase precursor

Summary Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

PDB	Organism	Resolution	Comment
1PNK	Escherichia coli	1.90 Å	complex with calcium
The active enzyme is shown. The structural calcium ions are shown as yellow CPK spheres. The catalytic Ser290 of the B-chain is shown in ball-and-stick representation in orange.

		Show surface

TERTIARY STRUCTURE DATA
Comment	Resolution	PDB	PDBe	SCOP	CATH	PDBSum	Proteopedia	Reference
Alcaligenes faecalis
thermostable penicillin g acylase from alcaligenes faecalis in orthorhombic form	3.31 Å	3K3W	3K3W	3K3W	3K3W	3K3W	3K3W
thermostable penicillin g acylase from alcaligenes faecalis in tetragonal form	3.50 Å	3ML0	3ML0	3ML0	3ML0	3ML0	3ML0
Bacillus megaterium
crystal structure of penicillin g acylase from bacillus megaterium	2.20 Å	6NVW	6NVW	6NVW	6NVW	6NVW	6NVW
Escherichia coli
complex with 3,4-dihydroxyphenylacetic acid	2.35 Å	1AI4	1AI4	1AI4	1AI4	1AI4	1AI4	Done et al., 1998
complex with m-nitrophenylacetic acid	2.36 Å	1AI5	1AI5	1AI5	1AI5	1AI5	1AI5	Done et al., 1998
complex with p-hydroxyphenylacetic acid	2.55 Å	1AI6	1AI6	1AI6	1AI6	1AI6	1AI6	Done et al., 1998
complex with phenol	2.50 Å	1AI7	1AI7	1AI7	1AI7	1AI7	1AI7	Done et al., 1998
complex with p-nitrophenylacetic acid	2.36 Å	1AJN	1AJN	1AJN	1AJN	1AJN	1AJN	Done et al., 1998
complex with 2,5-dihydroxyphenylacetic acid	2.31 Å	1AJP	1AJP	1AJP	1AJP	1AJP	1AJP	Done et al., 1998
complex with thiopheneacetic acid	2.05 Å	1AJQ	1AJQ	1AJQ	1AJQ	1AJQ	1AJQ	Done et al., 1998
slow processing precursor	1.80 Å	1E3A	1E3A	1E3A	1E3A	1E3A	1E3A	Hewitt et al., 2000
mutant; complex with phenylacetic acid	1.97 Å	1FXH	1FXH	1FXH	1FXH	1FXH	1FXH	Alkema et al., 2000
mutant; complex with penicillin G	2.25 Å	1FXV	1FXV	1FXV	1FXV	1FXV	1FXV	Alkema et al., 2000
complex with substrate	1.30 Å	1GK9	1GK9	1GK9	1GK9	1GK9	1GK9	McVey et al., 2001
complex with substrate	1.41 Å	1GKF	1GKF	1GKF	1GKF	1GKF	1GKF	McVey et al., 2001
complex with substrate	1.45 Å	1GM7	1GM7	1GM7	1GM7	1GM7	1GM7	McVey et al., 2001
complex with substrate	2.00 Å	1GM8	1GM8	1GM8	1GM8	1GM8	1GM8	McVey et al., 2001
complex with substrate	1.80 Å	1GM9	1GM9	1GM9	1GM9	1GM9	1GM9	McVey et al., 2001
altered substrate specificity mutant of penicillin acylase	2.00 Å	1H2G	1H2G	1H2G	1H2G	1H2G	1H2G	Morillas et al., 2003
penicillin acylase, mutant	2.28 Å	1JX9	1JX9	1JX9	1JX9	1JX9	1JX9	Alkema et al., 2004
penicillin acylase, mutant complexed with paa	2.34 Å	1K5Q	1K5Q	1K5Q	1K5Q	1K5Q	1K5Q	Alkema et al., 2004
penicillin acylase, mutant complexed with ppa	2.43 Å	1K5S	1K5S	1K5S	1K5S	1K5S	1K5S	Alkema et al., 2004
penicillin acylase mutant with phenyl proprionic acid	2.15 Å	1K7D	1K7D	1K7D	1K7D	1K7D	1K7D	Alkema et al., 2004
mutant; complex with phenyl proprionic acid	2.30 Å	1KEC	1KEC	1KEC	1KEC	1KEC	1KEC	Alkema et al., 2004
complex with calcium	1.90 Å	1PNK	1PNK	1PNK	1PNK	1PNK	1PNK	Duggleby et al., 1995
complex with 2-phenylacetic acid and calcium	2.50 Å	1PNL	1PNL	1PNL	1PNL	1PNL	1PNL	Duggleby et al., 1995
complex with benzylsulfinic acid and calcium	2.50 Å	1PNM	1PNM	1PNM	1PNM	1PNM	1PNM	Duggleby et al., 1995
Kluyvera citrophila
mature peptidase	2.80 Å	4PEL	4PEL	4PEL	4PEL	4PEL	4PEL
Providencia rettgeri
mature peptidase	2.50 Å	1CP9	1CP9	1CP9	1CP9	1CP9	1CP9	McDonough et al., 1999