Substrates for peptidase S01.153: elastase-1
Peptide and protein substrates that are thought to be physiologically relevant are indicated by P. Peptide and protein substrates that are thought to be pathologically relevant are indicated by D. Peptide and protein substrates that are not physiologically relevant are indicated by N. Synthetic substrates are indicated by S. Click on the symbol to show only physiological, non-physiological or synthetic substrates, or here to display all substrates. How cleavage sites have been identified are indicated by the following evidence codes: NT = N-terminal sequencing, MS = mass spectroscopy, MU = mutation, CS = consensus sequence, LC = liquid chromatography. To see all annotated cleavages for a protein substrate, click on the UniProt Accession.
| Substrate | Uniprot | Residue range | Cleavage Site | Cleavage type | Evidence | P4 | P3 | P2 | P1 | P1' | P2' | P3' | P4' | Reference | CutDB | MERNUM |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ac-Ala-Ala-Ala-OMe | Ac-Ala-Ala-Ala+OMe | S | Ac | Ala | Ala | Ala | OMe | - | - | - | Bieth, 2004 | |||||
| Ac-Ala-Pro-Ala-NHPhNO2 | Ac-Ala-Pro-Ala+NHPhNO2 | S | Ac | Ala | Pro | Ala | NAN | - | - | - | Bieth, 2004 | |||||
| alanyl bond | peptide-Ala+peptide | N | - | - | NPe | Ala | CPe | - | - | - | Bieth, 2004 | |||||
| alpha-2-macroglobulin | P01023 | 24-1474 | peptide-Val705+Gly-peptide | P | Gly | Leu | Arg | Val | Gly | Phe | Tyr | Glu | Mortensen et al., 1981 | |||
| alpha-2-macroglobulin | P01023 | 24-1474 | peptide-Gly706+Phe-peptide | P | Leu | Arg | Val | Gly | Phe | Tyr | Glu | Ser | Mortensen et al., 1981 | |||
| apolipoprotein(a) | P08519 | 20-4548 | peptide-Ile3539+Leu-peptide | P | Pro | Asn | Val | Ile | Leu | Ala | Pro | Ser | Edelstein et al., 1997 | 3308 | ||
| cAMP-dependent protein kinase type I-alpha regulatory subunit | P00514 | 2-46 | Ala+Ser-peptide | N | - | - | - | Ala | Ser | Gly | Thr | Thr | Titani et al., 1984 | |||
| cAMP-dependent protein kinase type I-alpha regulatory subunit | P00514 | 2-46 | peptide-Thr5+Thr-peptide | N | Ala | Ser | Gly | Thr | Thr | Ala | Ser | Glu | Titani et al., 1984 | |||
| elastin | peptide-Ala+Ala-peptide | P | - | - | NPe | Ala | Ala | CPe | - | - | Bieth, 2004 | |||||
| elastin | peptide-Ala+Gly-peptide | P | - | - | NPe | Ala | Gly | CPe | - | - | Bieth, 2004 | |||||
| endopin 2C | Q32T06 | 1-414 | peptide-Thr375+Ser-peptide | P | Ile | Met | Phe | Thr | Ser | Leu | Pro | Leu | Hwang et al., 2005 | |||
| Gly-Leu-Ala-Thr-Gly-Asn-Val-Ser-Thr-Ala-Glu-Leu-Gln-Asp-Ala-Thr-Pro-Ala-NHPhNO2 | Gly-Leu-Ala-Thr-Gly-Asn-Val-Ser-Thr-Ala-Glu-Leu-Gln-Asp-Ala-Thr-Pro-Ala+NHPhNO2 | S | Ala | Thr | Pro | Ala | NAN | - | - | - | Hauske et al., 2009 | |||||
| Hyaluronan and proteoglycan link protein 1 | P10915 | 16-354 | Peptide-His39+Leu-Peptide | N | NT | Asn | Gly | Pro | His | Leu | Leu | Val | Glu | Nguyen et al., 1991 | ||
| Hyaluronan and proteoglycan link protein 1 | P10915 | 16-354 | Peptide-Leu41+Val-Peptide | N | NT | Pro | His | Leu | Leu | Val | Glu | Ala | Glu | Nguyen et al., 1991 | ||
| insulin A chain (oxidized) | P01317 | 85-105 | peptide-Ala92+Ser-peptide | N | Gln | Cys | Cys | Ala | Ser | Val | Cys | Ser | Mei et al., 2003 | 24158 | ||
| insulin A chain (oxidized) | P01317 | 85-105 | peptide-Ser96+Leu-peptide | N | Ser | Val | Cys | Ser | Leu | Tyr | Gln | Leu | Mei et al., 2003 | 24159 | ||
| Insulin A chain (oxidized) | P01317 | 85-105 | Gly-Ile86+Val-Peptide | N | NT | - | - | Gly | Ile | Val | Glu | Gln | Cys | Naughton et al., 1961 | ||
| Insulin A chain (oxidized) | 85-105 | Gly-Ile-Val87+Glu-Peptide | N | NT | - | Gly | Ile | Val | Glu | Gln | Cys | Cys | Naughton et al., 1961 | |||
| Insulin A chain (oxidized) | P01317 | 85-105 | Peptide-Gln89+Cys-Peptide | N | NT | Ile | Val | Glu | Gln | Cys | Cys | Ala | Ser | Naughton et al., 1961 | ||
| Insulin A chain (oxidized) | P01317 | 85-105 | Peptide-Ser93+Val-Peptide | N | NT | Cys | Cys | Ala | Ser | Val | Cys | Ser | Leu | Naughton et al., 1961 | ||
| Insulin A chain (oxidized) | P01317 | 85-105 | Peptide-Leu97+Tyr-Peptide | N | NT | Val | Cys | Ser | Leu | Tyr | Gln | Leu | Glu | Naughton et al., 1961 | ||
| Insulin A chain (oxidized) | P01317 | 85-105 | Peptide-Tyr98+Gln-Peptide | N | NT | Cys | Ser | Leu | Tyr | Gln | Leu | Glu | Asn | Naughton et al., 1961 | ||
| Insulin A chain (oxidized) | P01317 | 85-105 | Peptide-Leu100+Glu-Peptide | N | NT | Leu | Tyr | Gln | Leu | Glu | Asn | Tyr | Cys | Naughton et al., 1961 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser+His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Leu | Cya | Gly | Ser | His | Leu | Val | Glu | Chen et al., 2009 | 24080 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val+Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Ser | His | Leu | Val | Glu | Ala | Leu | Tyr | Chen et al., 2009 | 24081 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala+Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Leu | Val | Glu | Ala | Leu | Tyr | Leu | Val | Chen et al., 2009 | 24082 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu+Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Ala | Leu | Tyr | Leu | Val | Cya | Gly | Glu | Bieth, 2004 | |||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val+Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Leu | Tyr | Leu | Val | Cya | Gly | Glu | Arg | Chen et al., 2009 | 24084 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly+Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Gly | Glu | Arg | Gly | Phe | Phe | Tyr | Thr | Chen et al., 2009 | 24077 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr+Thr-Pro-Lys-Ala | N | Gly | Phe | Phe | Tyr | Thr | Pro | Lys | Ala | Dumont et al., 1994 | 24076 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val+Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | - | - | Phe | Val | Asn | Gln | His | Leu | Dumont et al., 1994 | 24078 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu+Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Asn | Gln | His | Leu | Cya | Gly | Ser | His | Dumont et al., 1994 | 24079 | ||
| insulin B-chain (oxidized) | P01317 | 25-54 | Phe-Val-Asn-Gln-His-Leu-Cya-Gly-Ser-His-Leu-Val-Glu-Ala-Leu+Tyr-Leu-Val-Cya-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala | N | Val | Glu | Ala | Leu | Tyr | Leu | Val | Cya | Dumont et al., 1994 | 24083 | ||
| interleukin-1 beta precursor | P01584 | 1-269 | peptide-Ile103+Phe-peptide | P | Glu | Glu | Pro | Ile | Phe | Phe | Asp | Thr | Hazuda et al., 1990 | 19854 | ||
| leukocyte elastase inhibitor | P30740 | 1-379 | peptide-Cys344+Met-peptide | P | Ala | Thr | Phe | Cys | Met | Leu | Met | Pro | Cooley et al., 2001 | 20488 | ||
| Lys-Gly-Lys-Asn-Ser-Gly-Ser-Gly-Ala-Thr-Pro-Val-NHPhNO2 | Lys-Gly-Lys-Asn-Ser-Gly-Ser-Gly-Ala-Thr-Pro-Val-NHPhNO2 | S | Ala | Thr | Pro | Val | NAN | - | - | - | Hauske et al., 2009 | |||||
| Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val-NHPhNO2 | Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val+NHPhNO2 | S | Ala | Ser | Pro | Val | NAN | - | - | - | Hauske et al., 2009 | |||||
| Pro-Val-Phe-Asn-Thr-Leu-Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val-NHPhNO2 | Pro-Val-Phe-Asn-Thr-Leu-Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val+NHPhNO2 | S | Ala | Ser | Pro | Val | NAN | - | - | - | Hauske et al., 2009 | |||||
| regenerating islet-derived protein 3 alpha | Q06141 | 27-175 | peptide-Ser35+Ala-peptide | P | NT | Glu | Leu | Pro | Ser | Ala | Arg | Ile | Arg | Medveczky et al., 2009 | ||
| SCNN1G sodium channel, nonvoltage-gated 1 gamma | P51170 | 1-649 | peptide-Ala190+Ser-peptide | P | Ile | His | Lys | Ala | Ser | Asn | Val | Met | Passero et al., 2008 | 25559 | ||
| Ser-Gly-Arg-Val-Val-Pro-Gly-Tyr-Gly-His-Ala-NHPhNO2 | Ser-Gly-Arg-Val-Val-Pro-Gly-Tyr-Gly-His-Ala+NHPhNO2 | S | Tyr | Gly | His | Ala | NAN | - | - | - | Hauske et al., 2009 | |||||
| Ser-Pro-Leu-Pro-Glu-Gly-Val-NHPhNO2 | Ser-Pro-Leu-Pro-Glu-Gly-Val+NHPhNO2 | S | Pro | Glu | Gly | Val | NAN | - | - | - | Hauske et al., 2009 | |||||
| serpin {ECO:0000313|EMBL:AEW22118.1} | G8UQY8 | 1-432 | peptide-Thr386+Ala-peptide | P | NT | Ala | Ala | Val | Thr | Ala | Val | Glu | Met | Ksiazek et al., 2014 | ||
| serpin {ECO:0000313|EMBL:AEW22118.1} | G8UQY8 | 1-432 | peptide-Thr393+Ser-peptide | P | NT | Met | Val | Lys | Thr | Ser | Ser | Pro | Ser | Ksiazek et al., 2014 | ||
| serpin {ECO:0000313|EMBL:AEW22118.1} | G8UQY8 | 1-432 | peptide-Val391+Lys-peptide | P | NT | Val | Glu | Met | Val | Lys | Thr | Ser | Ser | Ksiazek et al., 2014 | ||
| Suc-Ala-Ala-Ala-Ala-NHPhNO2 | Suc-Ala-Ala-Ala-Ala+NHPhNO2 | S | Ala | Ala | Ala | Ala | NAN | - | - | - | Bieth, 2004 | |||||
| Suc-Ala-Ala-Ala-NHMec | Suc-Ala-Ala-Ala+NHMec | S | Suc | Ala | Ala | Ala | AMC | - | - | - | ||||||
| Suc-Ala-Ala-Ala-NHPhNO2 | Suc-Ala-Ala-Ala+NHPhNO2 | S | Suc | Ala | Ala | Ala | NAN | - | - | - | Bieth, 2004 | |||||
| Suc-Ala-Pro-Ala-NHPhNO2 | Suc-Ala-Pro-Ala+NHPhNO2 | S | Suc | Ala | Pro | Ala | NAN | - | - | - | ||||||
| tyrosyl bond | peptide-Tyr+peptide | N | - | - | NPe | Tyr | CPe | - | - | - | Bieth, 2004 | |||||
| Val-Phe-Asn-Thr-Leu-Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val-NHPhNO2 | Val-Phe-Asn-Thr-Leu-Pro-Met-Met-Gly-Lys-Ala-Ser-Pro-Val-NHPhNO2 | S | Ala | Ser | Pro | Val | NAN | - | - | - | Hauske et al., 2009 |
